ABSTRACT
Objective To investigate the effects of docosahexenoic acid (DHA) supplementation on infant's growth and BMI during pregnancy.Methods A total of 1 516 healthy pregnant women delivered their babies in two maternal and child health care hospitals in Beijing and were chosen as the subjects in this cohort study from May to October 2015.Self-developed questionnaires were used to gather general information of the subjects,including age,height,weight,weight gain during pregnancy,delivery mode,DHA supplementation etc.,before giving birth.Information on body length,weight,head circumference and BMI at birth and 6 months postnatal,of the infants were recorded.Breast milk was collected to test the fatty acid profiles by using the gas chromatography (GC) method at one to three months postnatally.Results The overall rate of DHA supplementation was 47.76％ among the pregnant women,in which introduction of DHA from the early and second stage of the pregnancy accounted for 49.31％ and 39.64％ respectively.When DHA supplementation began from the early pregnant stage,the DHA concentration showed an increase in the milk (P＜0.05),whereas the supplementation began from the second and third stages did not affect the milk DHA concentration (P＞0.05).Higher height and lower BMI were seen in the infants at birth and 6 months in the supplementation group when comparing to the non-supplementary group (P＜0.05),with the greatest effects noticed in the earliest supplementation group.Specifically,the head circumference appeared larger from the early pregnant stage in the DHA supplementary group,than that in the non-supplement group (P=0.001).The increment of head circumference was larger than that in the other groups when the infants were 6-month old (P＜0.01).Results from the partial regression analysis showed that during pregnancy,there were positive correlations between DHA supplementation and height (r=0.324,r=0.216),head circumference (r=0.221,r=0.302) as well as the increment of head circumference (r=0.276) at birth and 6 months (P＜0.05).Whereas,a negative correlation was shown between DHA and the infants' BMI (r=-0.310,r=-0.371) (P＜0.05) when supplementation was given during maternal pregnancy.Conclusions When DHA supplementation program was carried out during maternal pregnancy,it could increase the height and head circumference and inhibit the rapid increase of BMI in the infants BMI.Our findings seemed helpful in promoting brain development and preventing the childhood obesity.
ABSTRACT
Objective To investigate the effects of docosahexenoic acid (DHA) supplementation on infant's growth and BMI during pregnancy.Methods A total of 1 516 healthy pregnant women delivered their babies in two maternal and child health care hospitals in Beijing and were chosen as the subjects in this cohort study from May to October 2015.Self-developed questionnaires were used to gather general information of the subjects,including age,height,weight,weight gain during pregnancy,delivery mode,DHA supplementation etc.,before giving birth.Information on body length,weight,head circumference and BMI at birth and 6 months postnatal,of the infants were recorded.Breast milk was collected to test the fatty acid profiles by using the gas chromatography (GC) method at one to three months postnatally.Results The overall rate of DHA supplementation was 47.76％ among the pregnant women,in which introduction of DHA from the early and second stage of the pregnancy accounted for 49.31％ and 39.64％ respectively.When DHA supplementation began from the early pregnant stage,the DHA concentration showed an increase in the milk (P＜0.05),whereas the supplementation began from the second and third stages did not affect the milk DHA concentration (P＞0.05).Higher height and lower BMI were seen in the infants at birth and 6 months in the supplementation group when comparing to the non-supplementary group (P＜0.05),with the greatest effects noticed in the earliest supplementation group.Specifically,the head circumference appeared larger from the early pregnant stage in the DHA supplementary group,than that in the non-supplement group (P=0.001).The increment of head circumference was larger than that in the other groups when the infants were 6-month old (P＜0.01).Results from the partial regression analysis showed that during pregnancy,there were positive correlations between DHA supplementation and height (r=0.324,r=0.216),head circumference (r=0.221,r=0.302) as well as the increment of head circumference (r=0.276) at birth and 6 months (P＜0.05).Whereas,a negative correlation was shown between DHA and the infants' BMI (r=-0.310,r=-0.371) (P＜0.05) when supplementation was given during maternal pregnancy.Conclusions When DHA supplementation program was carried out during maternal pregnancy,it could increase the height and head circumference and inhibit the rapid increase of BMI in the infants BMI.Our findings seemed helpful in promoting brain development and preventing the childhood obesity.
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PURPOSE: To examine the effects of the oil mixes (ω-9, ω-6 and ω-3) in rats subjected to thermal burn. It was also aimed to assess whether the sources of ω3 would interfere with the effect of such mixes on the thermal injury. METHODS: Thirty-six rats distributed into five groups: burned + water, burned + isolipid mix, burned + oil mix 1 (ALA), burned + oil mix 2 (ALA + EPA + DHA of fish) and burned + oil mix 3 (ALA + DHA from seaweed). The thermal injury was involving total thickness of skin. After the burns animals received the oil mixes for seven days. The lesions were evaluated by immunohistochemistry. RESULTS: Animals receiving mix 3 showed a smaller extension of the thermal injury as compared to those that were supplemented with other oils mixes. Expression of Ki-67 in the receiving Mix 3 increased as compared to all the other groups. Animals supplemented with mix 3 were able to inhibit NF-κB in injured tissue. CONCLUSION: Rats received oil mix in which the source of ω3 (ALA+DHA of seaweed) showed inhibition of NF-κB, increase in cell proliferation, and reduction the extension of thermal lesion. .
Subject(s)
Animals , Male , Burns/drug therapy , Fatty Acids/pharmacology , /drug effects , NF-kappa B/drug effects , Seaweed/chemistry , Burns/pathology , Cell Proliferation/drug effects , Drug Combinations , /pharmacology , /therapeutic use , /pharmacology , /therapeutic use , Immunohistochemistry , /analysis , NF-kappa B/analysis , Random Allocation , Rats, Wistar , Reproducibility of Results , Time Factors , Treatment OutcomeABSTRACT
Objective To investigate the association between rs2281951 and rs3798753 in ELOVL fatty acid elongase2 (ELOVL2 gene)and the docosahexenoic acid (DHA)level in breast milk,and to clarify the influence of the polymorphisms of ELOVL2 gene in the DHA level of breast milk.Methods 209 healthy maternals were selected and signed the consent form and completed the 3-day 24-hour dietary recall questionaire on one day during the 22nd-the 25th day after partum,and 20 mL breast milk was collected.The DHA level in breast milk was detected with gas chromatography.The milk DNA was extracted and two single nucleotide polymorphisms (SNPs) of ELOVL2 gene were detected by Sequenom Mass Array System. UNPHASED 3.012 genetics software was adopted to analyze the quantitative trait of haplotype and the DHA level in breast milk.Results The distribution of genotypic frequencies of rs2281591 and rs3798713 sites in ELOVL2 gene was consistent with Hardy-Weinberg equilibrium (P > 0.05).The dietary fatty acid intakes and the milk DHA levels of maternals carrying different genotypes had no statistically significant differences (P > 0.05 ). The DHA levels in breast milk of maternals carring different rs3798713 (CG)-rs2281591 (AG)haplotypes had no statistically significant difference (χ2 =3.422,df =5,P =0.635).Conclusion Rs3798713 and rs2281951 and constructed haplotypes in ELOVL2 gene are not related to the DHA levels in breast milk.
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Objective To investigate the protection of DHA on the cisplatin cochlear injured modal animals ,and to explore the effect mechanism of DHA .Methods The animals were grouped randomly :control group ,DHA-treated group ,and cisplatin group .DHA-treated group at the doses of 500 mg?kg -1 ?d-1 .Control group and cisplatin group just treated vegetable oil at same doses ,all group′s treatment were lasting 30 day .Cisplatin intraperitoneal injection doses of 0 .25 mg/mL ,lasting 9 day ,begin with after DHA-treated 22 day .The intraperitoneal injections just do to DHA + cisplatin group and cisplatin group .Control group given in normal saline only .ABR was detected before the injection and after .Count the number of hair cells of cochlea after injection 9 day and detected the spiral ganglion of cochlea .Results After treatment ,ABR threshold of DHA-treated group was lower than that of the cisplatin group (P<0 .05) .DHA-treated group hair cell survival rate was significantly higher than that of cisplatin group(P<0 .05) .The spiral ganglion cells (SGCs) were severely damaged of cisplatin group than DHA-treated group .Conclusion DHA in-take may protect the cochlear from antioxidant injury of experiment animals ;these results may contribute to the treatments of cispl-atin caused deafness in clinic .
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Objective To study the effects of compound preparations which contain docosahexenoic acid (DHA),soybean lecithin,and vitamin A on memory ability of children and to compare the difference between two compound preparations that contain DHA from marine algae and from fish oil.Methods Totally 160 11-12-year-old healthy children who were studying in a primary school in Baoshan District of Shanghai were enrolled in this study.All the subjects signed the informed consent form.Subjects were randomly divided into three groups with random numbers:marine algae DHA group(n=53),fish oil DHA group(n=53),and control group(n=54).Subjects in the marine algae DHA group were given compound preparation which contained DHA form marine algae,soybean lecithin,and vitamin A;subjects in fish oil DHA group were given compound preparation which contained DHA form fish oil,soybean lecithin,and vitamin A.The dose of DHA(200 mg DHA per capsule)and other components in the two groups was equal.Subjects in the control group were given a placebo with same appearance.The trial lasted 30 days.Each subject took a capsule per day.Immediately before and after the trial,subjects were tested by using the clinical memory scale compiled by the Institute of Clinical Psychology of the Chinese Academy of Sciences.Results Before the trial.there was no difference amongthree groups in terms of all items of clinical memory scale or memory quotient(all P>0.05).After the trial,except for associative learning(both P>0.05),the other items of the clinical memory scale and memory quotient in both marine algae DHA group and fish oil DHA group were significantly higher than those of control group(all P<0.05).No significant difference was noted between the marine algae DHA group and fish oil DHA group for all items of the clinical memory scale or memory quotient(all P>0.05).Conclusions DHA compounds can impreve the memory ability of children.DHAs with different sources have similar effect on memory ability.
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In this paper,the method about hydrolysis of fish oil with lipases for concentrating DHA(docosahexenoic acid) in glycerides was studied.Six lipases were screened with comparing the activities of hydrolysis of fish oil.Candida lipalytica Lipase is resistant to generating DHA in fish oil.The Effects of the quantity of lipases,the emulgent,hydrolysis time,temperature in the process,the ratio of oil to water were studied.The optimum conditions for the hydrolysis of fish oil are 12 hours of hydrolysis under 45℃,with 300 u?g -1 .The emulgent was and the ratio of oil to water was 0.4 mL?g -1 .Under these conditions,the content of DHA in glyceride were concentrated from 18.7% to 34.0%.But the EPA content remained close to that of fish oil.The DHA glyceride containing triglyceride,diglyceride and monoglyceride was identified by IR spectrum combining with separation of HPLC.